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ready-to-use dapi staining solution  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science ready-to-use dapi staining solution
    Ready To Use Dapi Staining Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution(ready+to+use)/dapi+solution/pm39506528-56-37-43
    Average 90 stars, based on 1 article reviews
    ready-to-use dapi staining solution - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..

    Article Title: HUVECs’ Expressway Based on Magnesium Ion Doped Hierarchical Scaffold for Rapid Angiogenesis and Bone Ingrowth
    Article Snippet: Delayed union or nonunion remains an extremely challenging predicament even after the application of currently available engineering scaffolds for the treatment of critical-sized bone defects.. The angiogenesis throughout the artificial bone repair scaffold to achieve “angiogenic-osteogenic coupling” is regarded as an effective approach to solving this issue.. The newly formed blood vessels can accelerate the supply of oxygen and nutrients, thereby triggering subsequent bone regeneration.

    Article Title: Use of UMFNPs/Ce6@MBs in multimodal imaging-guided sono-photodynamic combination therapy for hepatocellular carcinoma.
    Article Snippet: Poly(ethylene glycol)-distearoylphosphatidylethanolamine (PEG-2000) and distearoylphosphatidylcholine (DSPC) were purchased from Avanti Inc. (USA); PEG2000-CRGD-NH2 was purchased from Xi’an Ruixi Biological Technology Co., Ltd; Ce6, DMEM, 0.25% trypsin (TE), fetal bovine serum (FBS), and 1% penicillin/streptomycin (1% penicillin and 1% streptomycin) were purchased from Gibco Inc. (USA); Huh-7 cells (FH0075 FuHeng Cell Center, Shanghai, China). .. The ROS fluorescent probe was purchased from Invitrogen Inc. (USA); phosphate-buffered saline (PBS) was purchased from Sigma-Aldrich Inc. (USA); carbon tetrachloride (CCl4) was purchased from Guangdong Huada Chemical Factory; 4% paraformaldehyde was purchased from Biosharp (China); ready-to-use DAPI staining solution was purchased from Solarbio (China); and the cytotoxicity assay kit (CCK-8) was purchased from Beijing Labgic Technology (China). ..

    Cell Characterization:

    Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..

    TUNEL Assay:

    Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..

    Immunohistochemistry:

    Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..

    Membrane:

    Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..

    Article Title: Triple Cross‐linked Dynamic Responsive Hydrogel Loaded with Selenium Nanoparticles for Modulating the Inflammatory Microenvironment via PI3K/Akt/NF‐κB and MAPK Signaling Pathways
    Article Snippet: .. Dextran T-70 (D8260), DAPI solution(ready-to-use) (C0065),LB Broth (powder, L1010), LB agar (powder, L1015), FBS, RIPA buffer (high) (R0010), BCA protein Assay kit (PC0020), and Matrigel Basement Membrane Matrix (356234) were purchased from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). .. Selenium dioxide (S817942), 2,2-Diphenyl-1-picrylhydrazyl (DPPH, D807297), 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, A800764), Ferrous sulfate (F903428), 4-(Bromomethyl)phenylboronic acid (B803441), Chitosan (C850347), and Sodium periodate (S817518) were acquire from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). ε-Poly-L-lysine٠HCl (P192512) was obtain from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China).

    Activity Assay:

    Article Title: MOF-818 Nanozyme Suppresses Calcium Oxalate Kidney Stones by Alleviating Oxidative Stress and Inflammatory Injury.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense chased from Wuhan Pricella Biotechnology Co., Ltd. Phorbol 12-myristate 13-acetate (PMA, HY-18739) was purchased from MedChemexpress (MCE); DAPI solution(ready to use) (C0065), Hematoxylin Eosin staining kit (G1120), and bovine serum protein BSA-V (A8020) were bought from Solarbio (Beijing, China); Cell Count Kit-8 (CCK-8, EK-5103) and Tissue fixing solution were purchased from ECOtop (ES-8101, Guangzhou, China); ROS detection kit (ROS, KGA7308-100) and cell cycle detection kit were purchased from KeyGEN Biotech (KGA9101-100, Nanjing, China); Tunel cell apoptosis detection kit (G1501, Servicebio, Wuhan, China); Immunohistochemistry UltraSensitiveTM SP kit (Maixin, China); Total Superoxide Dismutase Assay Kit with WST-8 (S0101S), Lyso-Tracker Red (C1046), Mitochondrial membrane potential detection kit (JC-1, C2006) and Calcein/PI cell activity and Cytotoxicity kit (C2015L) were acquired from Beyotime Biotechnology (Shanghai, China); The Quantikine Human TNF-α Immunoassay (DTA00D, R&D Systems, USA); Ultrasensitive S-P immunohistochemistry kit (EK-5103) and DAB solution (EK-5103) were purchased from Maixin Biotechnology (Fuzhou, China); DMEM/F12 (10 565 018), RPMI 1640 (11 875 500), trypsin EDTA (0.25%) containing phenol red (25 200 072) and PBS buffer (10 010 500) were bought from Gibco (Thermo Fisher scientific, USA); Fetal bovine serum (ST30-3302, PAN, Germany); Indocyanine Green (I953656-1 g), Trifluoroacetic acid (TFA, T818778), 1H-pyrazole-4-carboxylic acid (H2PyC, P816843-25 g), sodium oxalate (S818088), ammonium chloride (AC, S818088), and ethylene glycol (EG, S818088) were acquired from Macklin Biochemical (Shanghai, China); Zirconium oxychloride octahydrate (ZrOCl2 · 8H2O, 224316-5G) and N, N-dimethylformamide (DMF, D4551) were purchased from Sigma–Aldrich Corporation (Sigma, USA); Transwell 24-well plates (3421) and Transwell 6-well plates (3412) were purchased from Corning (Corning, USA)TIM-1 (ab78494, Abcam); SOD (67480-1-Ig, Proteintech); CAT (66765-1-Ig, Proteintech); OPN (22952-1-AP, Proteintech); CD44 (ab189524, Abcam); TNFα (60291-1-Ig, Proteintech); IL-6 (DF6087, Affinity); CD206 (24595s, CST); CD86 (sc-28347, Santa Cruz); Goat anti-Mouse lgG (#A-11005, Thermo scientific); Goat anti-Rabbit IgG (# A-11008, Thermo scientific). ..

    Labeling:

    Article Title: Peptide nano-blanket impedes fibroblasts activation and subsequent formation of pre-metastatic niche.
    Article Snippet: Cells were washed with PBS for three times and incubated for 1 h with AF647 labeled goat anti-rabbit IgG (H+ L) (1:100, Cat. 33113ES60, Yeasen, China). .. The nuclei were labeled by DAPI solution (ready-to-use) (Solarbio, China). .. Images were taken by confocal microscope (Leica, German).

    Saline:

    Article Title: Use of UMFNPs/Ce6@MBs in multimodal imaging-guided sono-photodynamic combination therapy for hepatocellular carcinoma.
    Article Snippet: Poly(ethylene glycol)-distearoylphosphatidylethanolamine (PEG-2000) and distearoylphosphatidylcholine (DSPC) were purchased from Avanti Inc. (USA); PEG2000-CRGD-NH2 was purchased from Xi’an Ruixi Biological Technology Co., Ltd; Ce6, DMEM, 0.25% trypsin (TE), fetal bovine serum (FBS), and 1% penicillin/streptomycin (1% penicillin and 1% streptomycin) were purchased from Gibco Inc. (USA); Huh-7 cells (FH0075 FuHeng Cell Center, Shanghai, China). .. The ROS fluorescent probe was purchased from Invitrogen Inc. (USA); phosphate-buffered saline (PBS) was purchased from Sigma-Aldrich Inc. (USA); carbon tetrachloride (CCl4) was purchased from Guangdong Huada Chemical Factory; 4% paraformaldehyde was purchased from Biosharp (China); ready-to-use DAPI staining solution was purchased from Solarbio (China); and the cytotoxicity assay kit (CCK-8) was purchased from Beijing Labgic Technology (China). ..

    Cytotoxicity Assay:

    Article Title: Use of UMFNPs/Ce6@MBs in multimodal imaging-guided sono-photodynamic combination therapy for hepatocellular carcinoma.
    Article Snippet: Poly(ethylene glycol)-distearoylphosphatidylethanolamine (PEG-2000) and distearoylphosphatidylcholine (DSPC) were purchased from Avanti Inc. (USA); PEG2000-CRGD-NH2 was purchased from Xi’an Ruixi Biological Technology Co., Ltd; Ce6, DMEM, 0.25% trypsin (TE), fetal bovine serum (FBS), and 1% penicillin/streptomycin (1% penicillin and 1% streptomycin) were purchased from Gibco Inc. (USA); Huh-7 cells (FH0075 FuHeng Cell Center, Shanghai, China). .. The ROS fluorescent probe was purchased from Invitrogen Inc. (USA); phosphate-buffered saline (PBS) was purchased from Sigma-Aldrich Inc. (USA); carbon tetrachloride (CCl4) was purchased from Guangdong Huada Chemical Factory; 4% paraformaldehyde was purchased from Biosharp (China); ready-to-use DAPI staining solution was purchased from Solarbio (China); and the cytotoxicity assay kit (CCK-8) was purchased from Beijing Labgic Technology (China). ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Triple Cross‐linked Dynamic Responsive Hydrogel Loaded with Selenium Nanoparticles for Modulating the Inflammatory Microenvironment via PI3K/Akt/NF‐κB and MAPK Signaling Pathways
    Article Snippet: .. Dextran T-70 (D8260), DAPI solution(ready-to-use) (C0065),LB Broth (powder, L1010), LB agar (powder, L1015), FBS, RIPA buffer (high) (R0010), BCA protein Assay kit (PC0020), and Matrigel Basement Membrane Matrix (356234) were purchased from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). .. Selenium dioxide (S817942), 2,2-Diphenyl-1-picrylhydrazyl (DPPH, D807297), 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, A800764), Ferrous sulfate (F903428), 4-(Bromomethyl)phenylboronic acid (B803441), Chitosan (C850347), and Sodium periodate (S817518) were acquire from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). ε-Poly-L-lysine٠HCl (P192512) was obtain from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China).



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